結合專利的去抑制因子技術,從各種已過濾水樣、空氣濾膜中提取出高質量基因組DNA
提取次數: |
貨號: |
50 次-不帶濾膜 |
14900-50-NF |
100 次-不帶濾膜 |
14900-100-NF |
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產品特點
- 專利IRT技術,擅長處理挑戰性的渾濁水樣
- 改進的裂解和濾膜DNA吸附技術,大大增加低微生物豐度水樣的DNA得率
- 創新研磨珠套管及擊打方式適應五種不同類型常用商業化濾膜
- 快速簡單的流水線式操作,輕松完成實驗
描述
PowerWater® DNA Isolation Kit是我們使用了IRT創新專利技術,適用于各種商業化濾膜的新一代水樣基因組總DNA提取試劑盒。有了這項IRT技術,即使污染物再多的水樣也能輕松提取得到高質量、可直接進行PCR實驗的大量DNA。你可以使用其它廠家的濾膜,也可以用我們含0.45μm 或 0.22 μm 濾膜的一次性漏斗(單獨有售)。此試劑盒使用了創新研磨珠套管及擊打方式結合IRT技術,可以直接處理47mm濾膜。優化過的處理程序也使得最終DNA體積可以縮小到100μl。
產品介紹彩頁
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英文原版說明書點擊下載
中文說明書下載
規格
方法 |
硅膠離心柱型 |
細胞破碎 |
研磨珠破碎 |
濾膜吸附能力 |
20μg 每孔 |
DNA純度
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A260/280: 1.7-2.0
A260/230: >1.5 |
通量 |
1 - 4 個樣 |
操作時間 |
20 分鐘 |
儀器設備 |
渦旋混合儀及適配器 |
水樣濾膜
- Jon J. Amberg, Sunnie G. McCalla, Loren Miller, Peter Sorensen and Mark P. Gaikowski. Detection of Environmental DNA of Bigheaded Carps in Samples Collected from Selected Locations in the St. Croix River and in the Mississippi River.USGS Open-File Report: 2013-1080
- Morgan M. Steffen, Zhou Li, T Chad Effler, Loren J. Hauser, Gregory L. Boyer, Steven W. Wilhelm. Comparative Metagenomics of Toxic Freshwater Cyanobacteria Bloom Communities on Two Continents. PLoS ONE 7(8): e44002. doi:10.1371/journal.pone.0044002
- Christopher L. Jerde, Andrew R. Mahon, W. Lindsay Chadderton, David M. Lodge. "Sight-unseen" detection of rare aquatic species using environmental DNA. Conservation Letters. Volume 4, Issue 2, pages 150–157, April/May 2011
- Van De Werfhorst*, L.C., Sercu, B., and Holden, P.A. Comparison of the Host Specificities of Two Bacteroidales Quantitative PCR Assays Used for Tracking Human Fecal Contamination. Applied and Environmental Microbiology. 77(17): 6258-6260 (2011).
空氣濾膜
- J. F. Meadow, A. E. Altrichter, S. W. Kembel, J. Kline, G. Mhuireach, M. Moriyama, D. Northcutt, T. K. O'Connor, A. M. Womack, G. Z. Brown, J. L . Green, B. J. M. Bohannan. Indoor airborne bacterial communities are influenced by ventilation, occupancy, and outdoor air source. Indoor Air. 24: 41–48 (2014)
- David J. Smith, Daniel A. Jaffe, Michele N. Birmele, DaleW. Griffin, Andrew C. Schuerger, Jonathan Hee, Michael S. Roberts. Free Tropospheric Transport of Microorganisms from Asia to North America. Microb Ecol. 64:973–985 (2012)
試劑盒組分
組分
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14900-50-NF
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Solution PW1 |
55 ml |
Solution PW2 |
11 ml |
Solution PW3 |
2 x 18 ml |
Solution PW4 |
2 x 18 ml |
Solution PW5 |
2 x 18 ml |
Solution PW6 |
5.5 ml |
PowerWater® Bead Tubes |
50 |
Spin Filters |
50 |
2 ml Collection Tubes |
250 |
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